Ionizable Lipid Nanoparticle-Mediated mRNA Delivery for Human CAR T Cell Engineering
University of Pennsylvania · Washington University in St. Louis · +1 more institution
Abstract
Chimeric antigen receptor (CAR) T cell therapy relies on the ex vivo manipulation of patient T cells to create potent, cancer-targeting therapies, shown to be capable of inducing remission in patients with acute lymphoblastic leukemia and large B cell lymphoma. However, current CAR T cell engineering methods use viral delivery vectors, which induce permanent CAR expression and could lead to severe adverse effects. Messenger RNA (mRNA) has been explored as a promising strategy for inducing transient CAR expression in T cells to mitigate the adverse effects associated with viral vectors, but it most commonly requires electroporation for T cell mRNA delivery, which can be cytotoxic. Here, ionizable lipid…
Citation impact
- FWCI
- 24.20
- Percentile
- 100%
- References
- 93
Authors
6Topics & keywords
- Jurkat cells
- Chimeric antigen receptor
- Electroporation
- Ex vivo
- Cytotoxicity
- Cancer cell
- Messenger RNA
- Chemistry
- Good health and well-being