articleScienceJan 23, 2020GREEN OA

Lineage tracing on transcriptional landscapes links state to fate during differentiation

CWCaleb WeinrebARAlejo Rodriguez-FraticelliFDFernando D. CamargoAMAllon M. Klein

Harvard University · Center for Systems Biology · +1 more institution

PubMed
Indexed incrossrefpubmed

Abstract

A challenge in biology is to associate molecular differences among progenitor cells with their capacity to generate mature cell types. Here, we used expressed DNA barcodes to clonally trace transcriptomes over time and applied this to study fate determination in hematopoiesis. We identified states of primed fate potential and located them on a continuous transcriptional landscape. We identified two routes of monocyte differentiation that leave an imprint on mature cells. Analysis of sister cells also revealed cells to have intrinsic fate biases not detectable by single-cell RNA sequencing. Finally, we benchmarked computational methods of dynamic inference from single-cell snapshots, showing that fate choice…

Citation impact

713
total citations
FWCI
34.92
Percentile
100%
References
36
Citations per year

Authors

4

Topics & keywords

Topics
Keywords
  • Biology
  • Cell fate determination
  • Cellular differentiation
  • Embryonic stem cell
  • Haematopoiesis
  • Stem cell
  • Progenitor cell
  • Gene
No related works found for this paper.

Funding