articleFrontiers in Bioengineering and BiotechnologyJan 22, 2020GOLD OA

Cryopreservation of Human iPS Cell Aggregates in a DMSO-Free Solution—An Optimization and Comparative Study

RLRui LiKHKathlyn HornbergerJRJames R. DuttonAHAllison Hubel

University of Minnesota

PubMed
Indexed incrossrefdoajpubmed

Abstract

Human induced pluripotent stem cells (hiPSCs) are an important cell source for regenerative medicine products. Effective methods of preservation are critical to their clinical and commercial applications. The use of a dimethyl sulfoxide (DMSO)-free solution containing all non-toxic molecules offers an effective alternative to the conventional DMSO and alleviates pain points associated with the use of DMSO in the cryopreservation of hiPSCs. Both hiPSCs and cells differentiated from them are commonly multicellular systems, which are more sensitive to stresses of freezing and thawing than single cells. In this investigation, low-temperature Raman spectroscopy visualized freezing behaviors of hiPSC aggregates in…

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Topics & keywords

Topics
Keywords
  • Cryopreservation
  • Dimethyl sulfoxide
  • Regenerative medicine
  • Chemistry
  • Induced pluripotent stem cell
  • Biophysics
  • Cell
  • Supercooling
UN Sustainable Development Goals
  • Life in Land
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