preprintbioRxiv (Cold Spring Harbor Laboratory)Jan 31, 2020GREEN OA

The novel coronavirus 2019 (2019-nCoV) uses the SARS-coronavirus receptor ACE2 and the cellular protease TMPRSS2 for entry into target cells

German Primate Center · University of Göttingen · +4 more institutions

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Abstract

Abstract The emergence of a novel, highly pathogenic coronavirus, 2019-nCoV, in China, and its rapid national and international spread pose a global health emergency. Coronaviruses use their spike proteins to select and enter target cells and insights into nCoV-2019 spike (S)-driven entry might facilitate assessment of pandemic potential and reveal therapeutic targets. Here, we demonstrate that 2019-nCoV-S uses the SARS-coronavirus receptor, ACE2, for entry and the cellular protease TMPRSS2 for 2019-nCoV-S priming. A TMPRSS2 inhibitor blocked entry and might constitute a treatment option. Finally, we show that the serum form a convalescent SARS patient neutralized 2019-nCoV-S-driven entry. Our results reveal…

Citation impact

757
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References
38
Citations per year

Authors

6

Topics & keywords

Keywords
  • Coronavirus
  • TMPRSS2
  • Virology
  • Pandemic
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
  • Coronavirus disease 2019 (COVID-19)
  • Severe acute respiratory syndrome coronavirus
  • Betacoronavirus
UN Sustainable Development Goals
  • Good health and well-being
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