articleJournal of Extracellular VesiclesFeb 3, 2020GOLD OA

MIFlowCyt‐EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

JAJoshua A WelshEVEdwin van der PolGJGer J. A. ArkesteijnMBMichel BremerABAlain Brisson

National Institutes of Health · National Cancer Institute · +23 more institutions

PubMed
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Abstract

Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and has produced a number of controversial results, in part due to limitations of instrument detection, lack of robust methods and ambiguities in how data should be interpreted. These complications are exacerbated by the field's lack of a robust reporting framework, and many EV-FC manuscripts include incomplete descriptions of methods and results, contain artefacts stemming from an insufficient instrument sensitivity and inappropriate experimental…

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Topics & keywords

Topics
Keywords
  • Extracellular vesicles
  • Standardization
  • Computer science
  • Extracellular vesicle
  • Flow cytometry
  • Sensitivity (control systems)
  • Measure (data warehouse)
  • Field (mathematics)
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