Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

Matsuyama, Shutoku; Nao, Naganori; Shirato, Kazuya; Kawase, Miyuki; Saito, Shinji; Takayama, Ikuyo; Nagata, Noriyo; Sekizuka, Tsuyoshi; Katoh, Hiroshi; Kato, Fumihiro; Sakata, Masafumi; Tahara, Maino; Kutsuna, Satoshi; Ohmagari, Norio; Kuroda, Makoto; Suzuki, Tadaki; Kageyama, Tsutomu; Takeda, Makoto

doi:10.1073/pnas.2002589117

articleProceedings of the National Academy of SciencesMar 12, 2020HYBRID OA

Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

SMShutoku Matsuyama NNNaganori Nao KSKazuya Shirato MKMiyuki Kawase SSShinji Saito

National Institute of Infectious Diseases · National Center for Global Health and Medicine

PubMed

Indexed incrossrefpubmed

Abstract

A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.

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1,628

total citations

FWCI: 34.10
Percentile: 100%
References: 15

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Authors

18

Topics & keywords

Topics

Keywords

TMPRSS2
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Isolation (microbiology)
Coronavirus disease 2019 (COVID-19)
2019-20 coronavirus outbreak
Virology
Sars virus
Betacoronavirus

UN Sustainable Development Goals

Good health and well-being

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