Transferrin Receptor Is a Specific Ferroptosis Marker

Ferroptosis is a type of regulated cell death driven by the iron-dependent accumulation of oxidized polyunsaturated fatty acid-containing phospholipids. There is no reliable way to selectively stain ferroptotic cells in tissue sections to characterize the extent of ferroptosis in animal models or patient samples. We address this gap by immunizing mice with membranes from lymphoma cells treated with the ferroptosis inducer piperazine erastin and screening ∼4,750 of the resulting monoclonal antibodies generated for their ability to selectively detect cells undergoing ferroptosis. We find that one antibody, 3F3 ferroptotic membrane antibody (3F3-FMA), is effective as a selective ferroptosis-staining reagent. The…

• MM
  M.J. Murdock Charitable Trust
• AA
  Arnold and Mabel Beckman Foundation

  Award: TR001875
• UO
  University of Miami

  Awards: P01CA087497, R35CA209896
• UO
  University of Pittsburgh
• UO
  University of Maryland
• UO
  University of Tokyo
• NI
  National Institutes of Health

  Award: P30 CA013696
• NC
  National Cancer Institute

  Awards: P01CA087497, R35CA209896, P30 CA013696
• NI
  National Institute of Neurological Disorders and Stroke

  Award: 1R61NS109407