articleClinical Infectious DiseasesMar 27, 2020BRONZE OA

Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients

Capital Medical University · Beijing Ditan Hospital · +3 more institutions

PubMed
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Abstract

Background

Coronavirus disease 2019 (COVID-19) has become a public health emergency. The widely used reverse transcription-polymerase chain reaction (RT-PCR) method has limitations for clinical diagnosis and treatment.

Methods

A total of 323 samples from 76 COVID-19-confirmed patients were analyzed by droplet digital PCR (ddPCR) and RT-PCR based 2 target genes (ORF1ab and N). Nasal swabs, throat swabs, sputum, blood, and urine were collected. Clinical and imaging data were obtained for clinical staging.

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Funding