Bradford Assay for Determining Protein Concentration
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Abstract
The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.
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Keywords
- Coomassie Brilliant Blue
- Absorbance
- Bradford protein assay
- Chromatography
- Chemistry
- Bicinchoninic acid assay
- Biochemistry
- Biology
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