articleCurrent Protocols in MicrobiologyApr 17, 2020HYBRID OA

SARS‐CoV‐2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup

DSDaniel StadlbauerFAFatima AmanatVCVeronika ChromikovaKJKaijun JiangSSShirin Strohmeier

Icahn School of Medicine at Mount Sinai · BOKU University

PubMed
Indexed incrossrefpubmed

Abstract

In late 2019, cases of atypical pneumonia were detected in China. The etiological agent was quickly identified as a betacoronavirus (named SARS-CoV-2), which has since caused a pandemic. Several methods allowing for the specific detection of viral nucleic acids have been established, but these only allow detection of the virus during a short period of time, generally during acute infection. Serological assays are urgently needed to conduct serosurveys, to understand the antibody responses mounted in response to the virus, and to identify individuals who are potentially immune to re-infection. Here we describe a detailed protocol for expression of antigens derived from the spike protein of SARS-CoV-2 that can…

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Topics & keywords

Topics
Keywords
  • Serology
  • Biology
  • Seroconversion
  • Virology
  • Antigen
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
  • Test (biology)
  • Coronavirus disease 2019 (COVID-19)
UN Sustainable Development Goals
  • Good health and well-being
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