Massively multiplexed nucleic acid detection with Cas13
Broad Institute · IIT@MIT · +6 more institutions
Abstract
Abstract The great majority of globally circulating pathogens go undetected, undermining patient care and hindering outbreak preparedness and response. To enable routine surveillance and comprehensive diagnostic applications, there is a need for detection technologies that can scale to test many samples 1–3 while simultaneously testing for many pathogens 4–6 . Here, we develop Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids (CARMEN), a platform for scalable, multiplexed pathogen detection. In the CARMEN platform, nanolitre droplets containing CRISPR-based nucleic acid detection reagents 7 self-organize in a microwell array 8 to pair with droplets of amplified samples, testing each…
Citation impact
- FWCI
- 41.51
- Percentile
- 100%
- References
- 39
Authors
18- CMCheri M. AckermanCorresponding
Broad Institute, IIT@MIT, Massachusetts Institute of Technology
- CMCameron Myhrvold
Broad Institute, Massachusetts Institute of Technology
- SGSri Gowtham Thakku
Broad Institute, Harvard University, Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology
- CACatherine A. Freije
Broad Institute, Harvard University, Massachusetts Institute of Technology
- HCHayden C. Metsky
Broad Institute, IIT@MIT, Massachusetts Institute of Technology
Topics & keywords
- Trans-activating crRNA
- CRISPR
- Nucleic acid
- Computational biology
- Nucleic acid detection
- Multiplexing
- Scalability
- Computer science
- Good health and well-being
Funding
- UDU.S. Department of Defense
- HHHoward Hughes Medical Institute
- KIKoch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
- NINational Institutes of HealthAward: F32CA236425
- DADefense Advanced Research Projects AgencyAward: D18AC00006
- DCDeshpande Center for Technological Innovation, Massachusetts Institute of Technology
- ARAdvanced Research Projects Agency