Site-specific glycan analysis of the SARS-CoV-2 spike
Centre for Human Genetics · University of Oxford · +2 more institutions
Abstract
The emergence of the betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), represents a considerable threat to global human health. Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, which mediates cell entry and membrane fusion. The SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in protein folding and immune evasion. Here, using a site-specific mass spectrometric approach, we reveal the glycan structures on a recombinant SARS-CoV-2 S immunogen. This analysis enables mapping of the glycan-processing states across the…
Citation impact
- FWCI
- 36.06
- Percentile
- 100%
- References
- 41
Authors
5- YWYasunori WatanabeCorresponding
Centre for Human Genetics, University of Oxford, University of Southampton
- JDJoel D. AllenCorresponding
University of Southampton
- DWDaniel Wrapp
The University of Texas at Austin
- JSJason S. McLellan
The University of Texas at Austin
- MCMax CrispinCorresponding
University of Southampton
Topics & keywords
- Glycan
- Epitope
- Glycosylation
- Spike (software development)
- Recombinant DNA
- Virology
- Neutralization
- Glycoprotein
- Good health and well-being