articleThe Science of The Total EnvironmentJun 20, 2020HYBRID OA

First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan

EHEiji HaramotoBMBikash MallaOTOcean ThakaliMKMasaaki Kitajima

Takeda (Japan) · University of Yamanashi · +1 more institution

PubMed
Indexed incrossrefpubmed

Abstract

Wastewater-based epidemiology is a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17…

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Topics & keywords

Topics
Keywords
  • Wastewater
  • RNA
  • RNA extraction
  • Extraction (chemistry)
  • Bovine coronavirus
  • Sewage treatment
  • Coronavirus
  • Feces
UN Sustainable Development Goals
  • Clean water and sanitation
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