Transport of Extracellular Vesicles across the Blood-Brain Barrier: Brain Pharmacokinetics and Effects of Inflammation
University of Puget Sound · University of Washington · +3 more institutions
Abstract
Extracellular vesicles can cross the blood-brain barrier (BBB), but little is known about passage. Here, we used multiple-time regression analysis to examine the ability of 10 exosome populations derived from mouse, human, cancerous, and non-cancerous cell lines to cross the BBB. All crossed the BBB, but rates varied over 10-fold. Lipopolysaccharide (LPS), an activator of the innate immune system, enhanced uptake independently of BBB disruption for six exosomes and decreased uptake for one. Wheatgerm agglutinin (WGA) modulated transport of five exosome populations, suggesting passage by adsorptive transcytosis. Mannose 6-phosphate inhibited uptake of J774A.1, demonstrating that its BBB transporter is the…
Citation impact
- FWCI
- 20.42
- Percentile
- 100%
- References
- 57
Authors
6- WAWilliam A. BanksCorresponding
University of Puget Sound, University of Washington, Geriatric Research Education and Clinical Center
- PSPriyanka Sharma
University of Pittsburgh, UPMC Hillman Cancer Center
- KMKristin M. Bullock
University of Washington
- KMKim M. Hansen
University of Washington
- NLNils Ludwig
University of Pittsburgh, UPMC Hillman Cancer Center
Topics & keywords
- Transcytosis
- Blood–brain barrier
- Microvesicles
- Exosome
- Cell biology
- Biology
- Transporter
- Vesicular transport protein
- Good health and well-being