Structures of Human Antibodies Bound to SARS-CoV-2 Spike Reveal Common Epitopes and Recurrent Features of Antibodies

And RBD epitopes on SARS-CoV-2 spike. Moreover, a 3.4 Å cryo-electron microscopy (cryo-EM) structure of a neutralizing monoclonal Fab-spike complex revealed an epitope that blocks ACE2 receptor binding. Modeling based on these structures suggested different potentials for inter-spike crosslinking by IgGs on viruses, and characterized IgGs would not be affected by identified SARS-CoV-2 spike mutations. Overall, our studies structurally define a recurrent anti-SARS-CoV-2 antibody class derived from VH3-53/VH3-66 and similarity to a SARS-CoV VH3-30 antibody, providing criteria for evaluating vaccine-elicited antibodies.

• HH
  Howard Hughes Medical Institute
• UD
  U.S. Department of Energy

  Awards: 76SF00515, P41GM103393, DE-AC02, AC02-76SF00515, DE-AC02-
• BA
  Bill and Melinda Gates Foundation

  Award: INV-002143
• GM
  George Mason University
• CI
  California Institute of Technology
• FG
  Fast Grants
• GC
  Georgia Clinical and Translational Science Alliance

  Award: UL1 TR001866
• NI
  National Institutes of Health

  Awards: P50 AI150464, P01-AI138938-S1, P41GM103393, AI138938, AI150464, TR001866, UL1 TR001866, DE-AC02-76SF00515, 2U19AI111825
• OO
  Office of Science

  Awards: AC02-76SF00515, P41GM103393, DE-AC02-76SF00515, DE-AC02, 76SF00515
• BI
  Beckman Institute, California Institute of Technology
• NI
  National Institute of General Medical Sciences

  Awards: P41GM103393, DE-AC02-76SF00515, AI150464
• NC
  National Center for Advancing Translational Sciences

  Awards: TR001866, UL1 TR001866
• BE
  Basic Energy Sciences

  Awards: DE-AC02, DE-AC02-76SF00515, P41GM103393, 76SF00515, AC02-76SF00515
• BA
  Biological and Environmental Research

  Awards: DE-AC02-76SF00515, P41GM103393
• SN
  SLAC National Accelerator Laboratory

  Awards: P41GM103393, AC02-76SF00515, DE-AC02-76SF00515