CRISPR-CasΦ from huge phages is a hypercompact genome editor
Innovative Genomics Institute · University of California, Berkeley · +7 more institutions
Abstract
CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive immunity against virus infection and plasmid transformation. We describe a minimal functional CRISPR-Cas system, comprising a single ~70-kilodalton protein, CasΦ, and a CRISPR array, encoded exclusively in the genomes of huge bacteriophages. CasΦ uses a single active site for both CRISPR RNA (crRNA) processing and crRNA-guided DNA cutting to target foreign nucleic acids. This hypercompact system is active in vitro and in human and plant cells with expanded target recognition capabilities relative to other CRISPR-Cas proteins. Useful for genome editing and DNA detection but with a molecular weight half that of Cas9 and Cas12a…
Citation impact
- FWCI
- 28.92
- Percentile
- 100%
- References
- 30
Authors
10- PPPatrick PauschCorresponding
Innovative Genomics Institute, University of California, Berkeley
- BABasem Al-ShayebCorresponding
Innovative Genomics Institute, University of California, Berkeley
- EBEzra Bisom-Rapp
University of California, Berkeley
- CAConnor A. Tsuchida
University of California, San Francisco, Innovative Genomics Institute, University of California, Berkeley
- ZLZheng Li
University of California, Los Angeles
Topics & keywords
- CRISPR
- Genome
- Genome editing
- Biology
- Computational biology
- RNA
- Gene
- Genetics