A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?
Research Manitoba · Children's Hospital Research Institute of Manitoba · +1 more institution
Abstract
Extracellular vesicles (EVs) are membranous vesicles secreted by both prokaryotic and eukaryotic cells and play a vital role in intercellular communication. EVs are classified into several subtypes based on their origin, physical characteristics, and biomolecular makeup. Exosomes, a subtype of EVs, are released by the fusion of multivesicular bodies (MVB) with the plasma membrane of the cell. Several methods have been described in literature to isolate exosomes from biofluids including blood, urine, milk, and cell culture media, among others. While differential ultracentrifugation (dUC) has been widely used to isolate exosomes, other techniques including ultrafiltration, precipitating agents such as…
Citation impact
- FWCI
- 30.59
- Percentile
- 100%
- References
- 121
Authors
3- KSKarim Sidhom
Research Manitoba, Children's Hospital Research Institute of Manitoba, University of Manitoba
- POPatience O. Obi
Research Manitoba, Children's Hospital Research Institute of Manitoba, University of Manitoba
- ASAyesha SaleemCorresponding
Research Manitoba, Children's Hospital Research Institute of Manitoba, University of Manitoba
Topics & keywords
- Microvesicles
- Exosome
- Extracellular vesicles
- Vesicle
- Ultrafiltration (renal)
- Nanoparticle tracking analysis
- Size-exclusion chromatography
- Computational biology
- Reduced inequalities