A large-scale binding and functional map of human RNA-binding proteins

. Here we introduce a new data set of RNA elements in the human genome that are recognized by RNA-binding proteins (RBPs), generated as part of the Encyclopedia of DNA Elements (ENCODE) project phase III. This class of regulatory elements functions only when transcribed into RNA, as they serve as the binding sites for RBPs that control post-transcriptional processes such as splicing, cleavage and polyadenylation, and the editing, localization, stability and translation of mRNAs. We describe the mapping and characterization of RNA elements recognized by a large collection of human RBPs in K562 and HepG2 cells. Integrative analyses using five assays identify RBP binding sites on RNA and chromatin in vivo, the in…

• NS
  National Science Foundation
• DR
  Damon Runyon Cancer Research Foundation

  Award: DRG-2172-13
• NI
  National Institutes of Health

  Awards: HG009530, R01HG004659, U54HG007005
• NH
  National Human Genome Research Institute

  Awards: U54HG007005, R01HG004659, HG009530, U41HG009889