The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an…

• AR
  Anglia Ruskin University
• EM
  European Molecular Biology Laboratory
• UH
  Universität Heidelberg
• IN
  Institut National de la Santé et de la Recherche Médicale
• UP
  Universität Potsdam
• UG
  Universiteit Gent
• BT
  Brandenburgische Technische Universität Cottbus-Senftenberg
• NI
  National Institute of Standards and Technology
• ER
  European Regional Development Fund
• DO
  Department of Pathology, University of Utah