A molecular pore spans the double membrane of the coronavirus replication organelle

Wolff, Georg; Limpens, Ronald W.A.L.; Zevenhoven-Dobbe, Jessika C.; Laugks, Ulrike; Zheng, Shawn; Jong, Anja W. M. de; Koning, Roman I.; Agard, David A.; Grünewald, Kay; Koster, Abraham J.; Snijder, Eric J.; Bárcena, Montserrat

doi:10.1126/science.abd3629

articleScienceAug 6, 2020HYBRID OA

A molecular pore spans the double membrane of the coronavirus replication organelle

GWGeorg Wolff RWRonald W.A.L. Limpens JCJessika C. Zevenhoven-Dobbe ULUlrike Laugks SZShawn Zheng

Leiden University Medical Center · Leibniz Institute of Virology (LIV) · +3 more institutions

PubMed

Indexed incrossrefpubmed

Abstract

Coronavirus genome replication is associated with virus-induced cytosolic double-membrane vesicles, which may provide a tailored microenvironment for viral RNA synthesis in the infected cell. However, it is unclear how newly synthesized genomes and messenger RNAs can travel from these sealed replication compartments to the cytosol to ensure their translation and the assembly of progeny virions. In this study, we used cellular cryo-electron microscopy to visualize a molecular pore complex that spans both membranes of the double-membrane vesicle and would allow export of RNA to the cytosol. A hexameric assembly of a large viral transmembrane protein was found to form the core of the crown-shaped complex. This…

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Topics & keywords

Topics

Keywords

Replication (statistics)
Organelle
Coronavirus
Cell biology
Chemistry
Virology
Biology
Coronavirus disease 2019 (COVID-19)

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