Single-nucleus transcriptome analysis reveals dysregulation of angiogenic endothelial cells and neuroprotective glia in Alzheimer’s disease
Hong Kong University of Science and Technology · University of Hong Kong · +1 more institution
Abstract
Significance Alzheimer’s disease (AD) has complex pathological mechanisms. Our limited understanding of the molecular and cellular changes in AD hindered the identification of therapeutic targets. In this study, we profiled 169,496 nuclei from the prefrontal cortical samples of AD patients and healthy controls by single-nucleus RNA sequencing. The results indicated that there is a loss of neuroprotective glial cells in AD, which may contribute to impaired neurotransmitter recycling in astrocytes, demyelination in oligodendrocytes, and perturbed synaptic pruning in microglia. Moreover, the analysis revealed a role of antigen presentation by angiogenic endothelial cells in AD. Together, our findings offer…
Citation impact
- FWCI
- 23.75
- Percentile
- 100%
- References
- 55
Authors
4- SLShun‐Fat LauCorresponding
Hong Kong University of Science and Technology, University of Hong Kong
- HCHan Cao
Hong Kong University of Science and Technology, University of Hong Kong
- AKAmy K.Y. Fu
Hong Kong University of Science and Technology, HKUST Shenzhen Research Institute, University of Hong Kong
- NYNancy Y. Ip
Hong Kong University of Science and Technology, HKUST Shenzhen Research Institute, University of Hong Kong
Topics & keywords
- Neuroprotection
- Transcriptome
- Neuroscience
- Disease
- Nucleus
- Angiogenesis
- Cell biology
- Biology
- Good health and well-being
Funding
- UGUniversity Grants CommitteeAward: AoE/M-604/16
- RGResearch Grants Council, University Grants CommitteeAwards: C6027-19GF, T13-607/12R, HKUST16149616, HKUST16103017, HKUST16102019, HKUST16100418
- IAInnovation and Technology CommissionAward: ITCPD/17-9
- SKShenzhen Knowledge Innovation ProgramAward: JCYJ20180507183642005