SARS-CoV-2 D614G variant exhibits efficient replication ex vivo and transmission in vivo

Hou, Yixuan J.; Chiba, S.; Halfmann, Peter; Ehré, Camille; Kuroda, Makoto; Dinnon, Kenneth H.; Leist, Sarah R.; Schäfer, Alexandra; Nakajima, Noriko; Takahashi, Kenta; Lee, Rhianna E.; Mascenik, Teresa; Graham, Rachel L.; Edwards, Caitlin E.; Tse, Longping V.; Okuda, Kenichi; Markmann, Alena J.; Bartelt, Luther A.; Silva, Aravinda de; Margolis, David M.; Boucher, Richard C.; Randell, Scott H.; Suzuki, Tadaki; Gralinski, Lisa E.; Kawaoka, Yoshihiro; Baric, Ralph S.

doi:10.1126/science.abe8499

articleScienceNov 12, 2020HYBRID OA

SARS-CoV-2 D614G variant exhibits efficient replication ex vivo and transmission in vivo

YJYixuan J. Hou SCS. Chiba PHPeter Halfmann CECamille Ehré MKMakoto Kuroda

University of North Carolina at Chapel Hill · University of Wisconsin–Madison · +2 more institutions

PubMed

Indexed incrossrefdatacitepubmed

Abstract

The spike aspartic acid-614 to glycine (D614G) substitution is prevalent in global severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains, but its effects on viral pathogenesis and transmissibility remain unclear. We engineered a SARS-CoV-2 variant containing this substitution. The variant exhibits more efficient infection, replication, and competitive fitness in primary human airway epithelial cells but maintains similar morphology and in vitro neutralization properties, compared with the ancestral wild-type virus. Infection of human angiotensin-converting enzyme 2 (ACE2) transgenic mice and Syrian hamsters with both viruses resulted in similar viral titers in respiratory tissues and pulmonary…

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Topics & keywords

Topics

Keywords

Biology
Virology
Ex vivo
Viral replication
Virus
In vivo
Infectivity
Nidovirales

UN Sustainable Development Goals

Good health and well-being

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