articleNature CommunicationsNov 11, 2020GOLD OA

The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

AYAisha YesbolatovaYSYuichiro SaitoNKNaomi KitamotoHMHatsune Makino‐ItouRARieko Ajima

National Institute of Genetics · The Graduate University for Advanced Studies, SOKENDAI · +4 more institutions

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Abstract

Protein knockdown using the auxin-inducible degron (AID) technology is useful to study protein function in living cells because it induces rapid depletion, which makes it possible to observe an immediate phenotype. However, the current AID system has two major drawbacks: leaky degradation and the requirement for a high dose of auxin. These negative features make it difficult to control precisely the expression level of a protein of interest in living cells and to apply this method to mice. Here, we overcome these problems by taking advantage of a bump-and-hole approach to establish the AID version 2 (AID2) system. AID2, which employs an OsTIR1(F74G) mutant and a ligand, 5-Ph-IAA, shows no detectable leaky…

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Topics & keywords

Topics
Keywords
  • Degron
  • Gene knockdown
  • Mutant
  • Cell biology
  • Yeast
  • Degradation (telecommunications)
  • Saccharomyces cerevisiae
  • Function (biology)
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