articleNature CommunicationsNov 20, 2020GOLD OA

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

JAJon Arizti-SanzCACatherine A. FreijeACAlexandra C. StantonBABrittany A. PetrosCKChloe K. Boehm

Broad Institute · Harvard–MIT Division of Health Sciences and Technology · +11 more institutions

PubMed
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Abstract

The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of Infections to Navigate Epidemics), a sensitive and specific diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We identify the optimal conditions to allow RPA-based amplification and Cas13-based detection to occur in a single step, simplifying assay preparation and reducing run-time. We improve HUDSON to rapidly inactivate viruses in nasopharyngeal swabs and saliva in 10 min. SHINE's results can be visualized with an in-tube fluorescent readout - reducing contamination risk as amplification reaction tubes remain…

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504
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23

Topics & keywords

Topics
Keywords
  • Coronavirus disease 2019 (COVID-19)
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
  • Transmission (telecommunications)
  • Pandemic
  • 2019-20 coronavirus outbreak
  • Saliva
  • Virology
  • Computer science
UN Sustainable Development Goals
  • Good health and well-being
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