Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer’s disease
Broad Institute · Columbia University Irving Medical Center · +6 more institutions
Abstract
The extent of microglial heterogeneity in humans remains a central yet poorly explored question in light of the development of therapies targeting this cell type. Here, we investigate the population structure of live microglia purified from human cerebral cortex samples obtained at autopsy and during neurosurgical procedures. Using single cell RNA sequencing, we find that some subsets are enriched for disease-related genes and RNA signatures. We confirm the presence of four of these microglial subpopulations histologically and illustrate the utility of our data by characterizing further microglial cluster 7, enriched for genes depleted in the cortex of individuals with Alzheimer's disease (AD). Histologically,…
Citation impact
- FWCI
- 34.03
- Percentile
- 100%
- References
- 43
Authors
27- MOMarta OlahCorresponding
Broad Institute, Columbia University Irving Medical Center
- VMVilas Menon
Broad Institute, Columbia University Irving Medical Center
- NHNaomi Habib
Broad Institute, Hebrew University of Jerusalem
- MTMariko Taga
Broad Institute, Columbia University Irving Medical Center
- YMYiyi Ma
Broad Institute, Columbia University Irving Medical Center
Topics & keywords
- Microglia
- Biology
- RNA
- Human brain
- Neurodegeneration
- Cell type
- Alzheimer's disease
- Disease