Single-cell RNA landscape of intratumoral heterogeneity and immunosuppressive microenvironment in advanced osteosarcoma

Zhou, Yan; Dong, Yang; Yang, Qingcheng; Lv, Xiaobin; Huang, Wentao; Zhou, Zhenhua; Wang, Yaling; Zhang, Zhichang; Yuan, Ting; Ding, Xiaomin; Tang, Lina; Zhang, Jianjun; Yin, Junyi; Huang, Yujing; Yu, Wenxi; Wang, Yonggang; Zhou, Chenliang; Yang, Su; He, Aina; Sun, Yuanjue; Shen, Zan; Qian, Bin‐Zhi; Meng, Wei; Fei, Jia; Yao, Yang; Pan, Xinghua; Chen, Peizhan; Hu, Haiyan

doi:10.1038/s41467-020-20059-6

articleNature CommunicationsDec 10, 2020GOLD OA

Single-cell RNA landscape of intratumoral heterogeneity and immunosuppressive microenvironment in advanced osteosarcoma

YZYan Zhou YDYang Dong QYQingcheng Yang XLXiaobin Lv WHWentao Huang

Shanghai Jiao Tong University · Shanghai Sixth People's Hospital · +10 more institutions

PubMed

Indexed incrossrefdoajpubmed

Abstract

Abstract Osteosarcoma is the most frequent primary bone tumor with poor prognosis. Through RNA-sequencing of 100,987 individual cells from 7 primary, 2 recurrent, and 2 lung metastatic osteosarcoma lesions, 11 major cell clusters are identified based on unbiased clustering of gene expression profiles and canonical markers. The transcriptomic properties, regulators and dynamics of osteosarcoma malignant cells together with their tumor microenvironment particularly stromal and immune cells are characterized. The transdifferentiation of malignant osteoblastic cells from malignant chondroblastic cells is revealed by analyses of inferred copy-number variation and trajectory. A proinflammatory FABP4 + macrophages…

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674

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28

Topics & keywords

Topics

Keywords

Osteosarcoma
Tumor microenvironment
Cancer research
RNA
Cell
Computational biology
Biology
Gene

UN Sustainable Development Goals

Good health and well-being

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