The Annals of Internal Medicine

Whole-exome sequencing and neoantigen prediction were performed as previously reported [12].For the immunotherapies, PBMCs were obtained from the patient using leukapheresis, and then prepared activated T lymphocytes or neoantigen peptide-pulsed DCs were prepared following the protocols [8,9,12].Genomic and neoantigen analyses, cell processing, and immunotherapy