articleCellJan 1, 2022HYBRID OA

Parallel analysis of transcription, integration, and sequence of single HIV-1 proviruses

KBKevin B. EinkaufMRMatthew R. OsbornCGCe GaoWSWeiwei SunXSXiaoming Sun

Ragon Institute of MGH, MIT and Harvard · San Francisco VA Medical Center · +1 more institution

PubMed
Indexed incrossrefpubmed

Abstract

HIV-1-infected cells that persist despite antiretroviral therapy (ART) are frequently considered "transcriptionally silent," but active viral gene expression may occur in some cells, challenging the concept of viral latency. Applying an assay for profiling the transcriptional activity and the chromosomal locations of individual proviruses, we describe a global genomic and epigenetic map of transcriptionally active and silent proviral species and evaluate their longitudinal evolution in persons receiving suppressive ART. Using genome-wide epigenetic reference data, we show that proviral transcriptional activity is associated with activating epigenetic chromatin features in linear proximity of integration sites…

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Authors

15
  • KB
    Kevin B. EinkaufCorresponding

    Ragon Institute of MGH, MIT and Harvard

  • MR
    Matthew R. Osborn

    Ragon Institute of MGH, MIT and Harvard

  • CG
    Ce Gao

    Ragon Institute of MGH, MIT and Harvard

  • WS
    Weiwei Sun

    Ragon Institute of MGH, MIT and Harvard

  • XS
    Xiaoming Sun

    Ragon Institute of MGH, MIT and Harvard

Topics & keywords

Topics
Keywords
  • Epigenetics
  • Chromatin
  • Gene
  • Genome
  • Selection (genetic algorithm)
  • Gene expression profiling
  • Negative selection
  • Viral replication
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