Multi-kingdom microbiota analyses identifybacterial–fungal interactions and biomarkers of colorectal cancer acrosscohorts

Despite recent progress in our understanding of the association between the gut microbiome and colorectal cancer (CRC), multi-kingdom gut microbiome dysbiosis in CRC across cohorts is unexplored. We investigated four-kingdom microbiota alterations using CRC metagenomic datasets of 1,368 samples from 8 distinct geographical cohorts. Integrated analysis identified 20 archaeal, 27 bacterial, 20 fungal and 21 viral species for each single-kingdom diagnostic model. However, our data revealed superior diagnostic accuracy for models constructed with multi-kingdom markers, in particular the addition of fungal species. Specifically, 16 multi-kingdom markers including 11 bacterial, 4 fungal and 1 archaeal feature,…

• CI
  Centre International de Recherche sur le Cancer
• NN
  National Natural Science Foundation of China

  Award: 31900129
• CP
  China Postdoctoral Science Foundation

  Awards: 2017SHZDZX01, 2019M663252, 2019M651568
• SJ
  Shanghai Jiao Tong University

  Awards: YG2020YQ19, 2020YFA0907200
• NP
  National Postdoctoral Program for Innovative Talents

  Awards: 2019M663252, BX20190393