articleRedox BiologyFeb 9, 2022GOLD OA

ACSL4 deficiency confers protection against ferroptosis-mediated acute kidney injury

YWYue WangMZMenghan ZhangRBRan BiYSYali SuFQFei Quan

China Pharmaceutical University · Anhui Medical University · +2 more institutions

PubMed
Indexed incrossrefdoajpubmed

Abstract

The term ferroptosis coined in 2012 causes acute kidney injury (AKI). However, its pathway mechanism in AKI is poorly understood. In this study, we conducted an RNA-sequence analysis of kidneys in AKI and normal mice to explore the pathway mechanism of ferroptosis. Consequently, differentially expressed genes highlighted Acyl-CoA synthetase long-chain family (ACSL4), a known promotor for ferroptosis. Besides, RT-PCR, Western blot, and immunohistochemical analyses confirmed its upregulation. HIF-1α was downregulated in I/R-AKI mice, and in vitro studies confirmed a negative regulation of HIF-1α on ACSL4. To explore the role of ACSL4 in AKI, we constructed ACSL4 knockout in kidney tubules of mice-as…

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401
total citations
FWCI
55.15
Percentile
100%
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66
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Authors

14

Topics & keywords

Topics
Keywords
  • Acute kidney injury
  • Kidney
  • Pathogenesis
  • Downregulation and upregulation
  • Cancer research
  • Knockout mouse
  • Inflammation
  • Biology
UN Sustainable Development Goals
  • Good health and well-being
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