Current applications and future perspective of CRISPR/Cas9 gene editing in cancer

Clustered regularly interspaced short palindromic repeats (CRISPR) system provides adaptive immunity against plasmids and phages in prokaryotes. This system inspires the development of a powerful genome engineering tool, the CRISPR/CRISPR-associated nuclease 9 (CRISPR/Cas9) genome editing system. Due to its high efficiency and precision, the CRISPR/Cas9 technique has been employed to explore the functions of cancer-related genes, establish tumor-bearing animal models and probe drug targets, vastly increasing our understanding of cancer genomics. Here, we review current status of CRISPR/Cas9 gene editing technology in oncological research. We first explain the basic principles of CRISPR/Cas9 gene editing and…

• NS
  Natural Science Foundation of Shanghai

  Awards: 22ZR1411200, 21ZR1412200
• HM
  Harbin Medical University
• NN
  National Natural Science Foundation of China

  Awards: 21ZR1412200, 81672825, SHDC2020CR2067B, 81602513
• FU
  Fudan University
• SJ
  Shanghai Jiao Tong University