Ultra‐high sensitivity mass spectrometry quantifies single‐cell proteome changes upon perturbation
Max Planck Institute of Biochemistry · University of Copenhagen · +6 more institutions
Abstract
Single-cell technologies are revolutionizing biology but are today mainly limited to imaging and deep sequencing. However, proteins are the main drivers of cellular function and in-depth characterization of individual cells by mass spectrometry (MS)-based proteomics would thus be highly valuable and complementary. Here, we develop a robust workflow combining miniaturized sample preparation, very low flow-rate chromatography, and a novel trapped ion mobility mass spectrometer, resulting in a more than 10-fold improved sensitivity. We precisely and robustly quantify proteomes and their changes in single, FACS-isolated cells. Arresting cells at defined stages of the cell cycle by drug treatment retrieves expected…
Citation impact
- FWCI
- 52.89
- Percentile
- 100%
- References
- 67
Authors
17Topics & keywords
- Proteome
- Biology
- Proteomics
- Transcriptome
- Mass spectrometry
- Computational biology
- Single-cell analysis
- Systems biology
- Good health and well-being