articleNature BiotechnologyMar 3, 2022HYBRID OA

Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells

DBDiego BalboaTBTom BarsbyVLVäinö LithoviusJSJonna Saarimäki‐VireMOMuhmmad Omar‐Hmeadi

University of Helsinki · Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas · +6 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Transplantation of pancreatic islet cells derived from human pluripotent stem cells is a promising treatment for diabetes. Despite progress in the generation of stem-cell-derived islets (SC-islets), no detailed characterization of their functional properties has been conducted. Here, we generated functionally mature SC-islets using an optimized protocol and benchmarked them comprehensively against primary adult islets. Biphasic glucose-stimulated insulin secretion developed during in vitro maturation, associated with cytoarchitectural reorganization and the increasing presence of alpha cells. Electrophysiology, signaling and exocytosis of SC-islets were similar to those of adult islets. Glucose-responsive…

Citation impact

403
total citations
FWCI
84.20
Percentile
100%
References
96
Citations per year

Authors

25

Topics & keywords

Topics
Keywords
  • Islet
  • Pancreatic islets
  • Biology
  • Cell biology
  • Transplantation
  • Transcriptome
  • Induced pluripotent stem cell
  • Stem cell
UN Sustainable Development Goals
  • Good health and well-being
No related works found for this paper.

Funding