Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids

Matrigel, a mouse tumor extracellular matrix protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility of organoids for drug development and regenerative medicine due to its tumor-derived origin, batch-to-batch variation, high cost, and safety issues. Here, we demonstrate that gastrointestinal tissue-derived extracellular matrix hydrogels are suitable substitutes for Matrigel in gastrointestinal organoid culture. We found that the development and function of gastric or intestinal organoids grown in tissue extracellular matrix hydrogels are comparable or often superior to those in Matrigel. In addition, gastrointestinal extracellular matrix hydrogels…

• NR
  National Research Foundation
• YU
  Yonsei University

  Award: 2021-22-0014
• NR
  National Research Foundation of Korea

  Awards: IBS-R026-D1, 2021R1A2C3004262, 2018M3A9H1021382
• IF
  Institute for Basic Science

  Awards: IBS-R026-D1, R026-D1
• MO
  Ministry of Science and ICT, South Korea

  Awards: IBS-R026-D1, 20009125
• KE
  Korea Evaluation Institute of Industrial Technology

  Award: 20009125