Neutrophil extracellular traps regulate ischemic stroke brain injury

Ischemic stroke prompts a strong inflammatory response, which is associated with exacerbated outcomes. In this study, we investigated mechanistic regulators of neutrophil extracellular trap (NET) formation in stroke and whether they contribute to stroke outcomes. NET-forming neutrophils were found throughout brain tissue of ischemic stroke patients, and elevated plasma NET biomarkers correlated with worse stroke outcomes. Additionally, we observed increased plasma and platelet surface-expressed high-mobility group box 1 (HMGB1) in stroke patients. Mechanistically, platelets were identified as the critical source of HMGB1 that caused NETs in the acute phase of stroke. Depletion of platelets or platelet-specific…

• AH
  American Heart Association

  Award: 2021Post830138
• NI
  National Institutes of Health

  Awards: R01HL163019, R35GM119526, K01AG059892
• NI
  National Institute on Aging

  Award: K01AG059892
• NH
  National Heart, Lung, and Blood Institute

  Award: R01HL163019
• NI
  National Institute of General Medical Sciences

  Award: R35GM119526
• NI
  National Institute of Neurological Disorders and Stroke

  Award: U24NS107228
• NC
  National Center for Research Resources

  Award: 1S10OD026959-01
• EK
  Eunice Kennedy Shriver National Institute of Child Health and Human Development

  Award: R01093826