A functional corona around extracellular vesicles enhances angiogenesis, skin regeneration and immunomodulation
Paracelsus Medical University · University of Salzburg · +6 more institutions
Abstract
Nanoparticles can acquire a plasma protein corona defining their biological identity. Corona functions were previously considered for cell-derived extracellular vesicles (EVs). Here we demonstrate that nano-sized EVs from therapy-grade human placental-expanded (PLX) stromal cells are surrounded by an imageable and functional protein corona when enriched with permissive technology. Scalable EV separation from cell-secreted soluble factors via tangential flow-filtration (TFF) and subtractive tandem mass-tag (TMT) proteomics revealed significant enrichment of predominantly immunomodulatory and proangiogenic proteins. Western blot, calcein-based flow cytometry, super-resolution and electron microscopy verified EV…
Citation impact
- FWCI
- 19.66
- Percentile
- 100%
- References
- 52
Authors
22Topics & keywords
- Angiogenesis
- Cell biology
- Chemistry
- Exosome
- Flow cytometry
- Microvesicles
- Biophysics
- Biology