articleScienceApr 7, 2022GREEN OA

Single-cell eQTL mapping identifies cell type–specific genetic control of autoimmune disease

Garvan Institute of Medical Research · The University of Queensland · +10 more institutions

PubMed
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Abstract

The human immune system displays substantial variation between individuals, leading to differences in susceptibility to autoimmune disease. We present single-cell RNA sequencing (scRNA-seq) data from 1,267,758 peripheral blood mononuclear cells from 982 healthy human subjects. For 14 cell types, we identified 26,597 independent cis-expression quantitative trait loci (eQTLs) and 990 trans-eQTLs, with most showing cell type-specific effects on gene expression. We subsequently show how eQTLs have dynamic allelic effects in B cells that are transitioning from naïve to memory states and demonstrate how commonly segregating alleles lead to interindividual variation in immune function. Finally, using a Mendelian…

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Authors

17

Topics & keywords

Keywords
  • Expression quantitative trait loci
  • Mendelian randomization
  • Biology
  • Immune system
  • Genome-wide association study
  • Allele
  • Genetics
  • Autoimmune disease
UN Sustainable Development Goals
  • Good health and well-being
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