Single cell transcriptomic landscape of diabetic foot ulcers
Beth Israel Deaconess Medical Center · Emory University · +6 more institutions
Abstract
Diabetic foot ulceration (DFU) is a devastating complication of diabetes whose pathogenesis remains incompletely understood. Here, we profile 174,962 single cells from the foot, forearm, and peripheral blood mononuclear cells using single-cell RNA sequencing. Our analysis shows enrichment of a unique population of fibroblasts overexpressing MMP1, MMP3, MMP11, HIF1A, CHI3L1, and TNFAIP6 and increased M1 macrophage polarization in the DFU patients with healing wounds. Further, analysis of spatially separated samples from the same patient and spatial transcriptomics reveal preferential localization of these healing associated fibroblasts toward the wound bed as compared to the wound edge or unwounded skin.…
Citation impact
- FWCI
- 48.58
- Percentile
- 100%
- References
- 98
Authors
19- GTGeorgios TheocharidisCorresponding
Beth Israel Deaconess Medical Center
- BTBeena Thomas
Emory University, Children's Healthcare of Atlanta
- DSDebasree Sarkar
Emory University, Children's Healthcare of Atlanta
- HMHope Mumme
Emory University, Children's Healthcare of Atlanta
- WPWilliam Pilcher
Emory University, Children's Healthcare of Atlanta
Topics & keywords
- Wound healing
- Diabetic foot
- Transcriptome
- Population
- Medicine
- Cell
- Diabetes mellitus
- Peripheral blood mononuclear cell
- Good health and well-being