Best practices in metabarcoding of fungi: From experimental design to results
King Saud University · University of Tartu · +11 more institutions
Abstract
The development of high-throughput sequencing (HTS) technologies has greatly improved our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems. Here we provide an overview of current best practices in metabarcoding analysis of fungal communities, from experimental design through molecular and computational analyses. By reanalysing published data sets, we demonstrate that operational taxonomic units (OTUs) outperform amplified sequence variants (ASVs) in recovering fungal diversity, a finding that is particularly evident for long markers. Additionally, analysis of the full-length ITS region allows more accurate taxonomic placement of fungi and other eukaryotes compared to…
Citation impact
- FWCI
- 61.11
- Percentile
- 100%
- References
- 293
Authors
8- LTLeho TedersooCorresponding
King Saud University, University of Tartu
- MBMohammad Bahram
Swedish University of Agricultural Sciences, University of Tartu
- LZLucie Zinger
Centre National de la Recherche Scientifique, Naturalis Biodiversity Center, Inserm, Université Paris Sciences et Lettres, École Normale Supérieure - PSL, Institut de Biologie de l'École Normale Supérieure
- RHR. Henrik Nilsson
University of Gothenburg
- PGPeter G. Kennedy
University of Minnesota
Topics & keywords
- Biology
- Context (archaeology)
- Fungal Diversity
- Ecosystem
- Ecology
- Evolutionary biology
- DNA barcoding
- Environmental DNA
- Life in Land