Single-cell genomic profiling of human dopamine neurons identifies a population that selectively degenerates in Parkinson’s disease
Broad Institute · Harvard University · +2 more institutions
Abstract
The loss of dopamine (DA) neurons within the substantia nigra pars compacta (SNpc) is a defining pathological hallmark of Parkinson's disease (PD). Nevertheless, the molecular features associated with DA neuron vulnerability have not yet been fully identified. Here, we developed a protocol to enrich and transcriptionally profile DA neurons from patients with PD and matched controls, sampling a total of 387,483 nuclei, including 22,048 DA neuron profiles. We identified ten populations and spatially localized each within the SNpc using Slide-seq. A single subtype, marked by the expression of the gene AGTR1 and spatially confined to the ventral tier of SNpc, was highly susceptible to loss in PD and showed the…
Citation impact
- FWCI
- 46.95
- Percentile
- 100%
- References
- 71
Authors
9- TKTushar Kamath
Broad Institute, Harvard University, Stanley Center for Psychiatric Research
- AAAbdulraouf Abdulraouf
Broad Institute, Stanley Center for Psychiatric Research
- SBS.J. Burris
Broad Institute, Stanley Center for Psychiatric Research
- JLJonah Langlieb
Broad Institute, Stanley Center for Psychiatric Research
- VGVahid Gazestani
Broad Institute, Stanley Center for Psychiatric Research
Topics & keywords
- Pars compacta
- Substantia nigra
- Neuroscience
- Biology
- Dopamine
- Population
- Parkinson's disease
- Neuron