Phosphatidylserine-dependent ingestion of apoptotic cells promotes TGF-β1 secretion and the resolution of inflammation
University of Colorado Health · National Jewish Health · +1 more institution
Abstract
Ingestion of apoptotic cells in vitro by macrophages induces TGF-1 secretion, resulting in an antiinflammatory effect and suppression of proinflammatory mediators.Here, we show in vivo that direct instillation of apoptotic cells enhanced the resolution of acute inflammation.This enhancement appeared to require phosphatidylserine (PS) on the apoptotic cells and local induction of TGF-1.Working with thioglycollate-stimulated peritonea or LPS-stimulated lungs, we examined the effect of apoptotic cell uptake on TGF-1 induction.Viable or opsonized apoptotic human Jurkat T cells, or apoptotic PLB-985 cells, human monomyelocytes that do not express PS during apoptosis, failed to induce TGF-1.PS liposomes, or PS…
Citation impact
- FWCI
- 14.94
- Percentile
- 100%
- References
- 50
Authors
3Topics & keywords
- Phosphatidylserine
- Apoptosis
- Proinflammatory cytokine
- Inflammation
- Jurkat cells
- Efferocytosis
- Chemokine
- Cell biology