Greener Journal of Biological Sciences

Item analysis is used to examine students' responses to items to determine the quality of an assessment tool.This study aimed at assessing the quality of the objective structured practical examination (OSPE) on Introductory Microbiology.41 first-year Microbiology major students of Bayelsa Medical University, Nigeria took part in the OSPE containing 40 items.Marks were not deducted for wrong answers as decided by the department and each item carried one mark, and 40% was the pass mark.The items were analyzed for the difficulty index, discrimination index, distractor efficiency, and reliability index (Cronbach's alpha).Also, key distributions, numbers, and percentage passed were determined.Results showed that…