articleAnalytical ChemistryMay 12, 2022Closed access

CRISPR-Cas12a-Derived Photoelectrochemical Biosensor for Point-Of-Care Diagnosis of Nucleic Acid

RZRuijin ZengHGHexiang GongYLYanli LiYLYuxuan LiWLWei Lin

Fuzhou University · Technical University of Munich

PubMed
Indexed incrossrefpubmed

Abstract

This work presented a point-of-care (POC) photoelectrochemical (PEC) biosensing for the detection of human papillomavirus-16 (HPV-16) on a portable electrochemical detection system by using CRISPR-Cas12a trans-cleaving the G-quadruplex for the biorecognition/amplification and a hollow In2O3–In2S3-modified screen-printed electrode (In2O3–In2S3/SPE) as the photoactive material. G-quadruplexes were capable of biocatalytic precipitation (H2O2-mediated 4-chloro-1-naphthol oxidation) on the In2O3–In2S3/SPE surface, resulting in a weakened photocurrent, but suffered from trans-cleavage when the CRISPR-Cas12a system specifically recognized the analyte. The photocurrent results could be directly observed with the…

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384
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Authors

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Topics & keywords

Topics
Keywords
  • Chemistry
  • Nucleic acid
  • Biosensor
  • CRISPR
  • Nucleic acid detection
  • Point of care
  • Nanotechnology
  • Molecular beacon
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