Lipid-Nanoparticle-Based Delivery of CRISPR/Cas9 Genome-Editing Components
University of British Columbia · BC Children's Hospital
Abstract
Gene editing mediated by CRISPR/Cas9 systems is due to become a beneficial therapeutic option for treating genetic diseases and some cancers. However, there are challenges in delivering CRISPR components which necessitate sophisticated delivery systems for safe and effective genome editing. Lipid nanoparticles (LNPs) have become an attractive nonviral delivery platform for CRISPR-mediated genome editing due to their low immunogenicity and application flexibility. In this review, we provide a background of CRISPR-mediated gene therapy, as well as LNPs and their applicable characteristics for delivering CRISPR components. We then highlight the challenges of CRISPR delivery, which have driven the significant…
Citation impact
- FWCI
- 20.01
- Percentile
- 100%
- References
- 99
Authors
6- PKPardis Kazemian
University of British Columbia, BC Children's Hospital
- SYSi-Yue Yu
University of British Columbia
- SBSarah B. Thomson
University of British Columbia, BC Children's Hospital
- ABAlexandra Birkenshaw
University of British Columbia
- BRBlair R. Leavitt
University of British Columbia, BC Children's Hospital
Topics & keywords
- CRISPR
- Genome editing
- Cas9
- Computational biology
- Flexibility (engineering)
- Genome
- Computer science
- Biology