Single-cell RNA sequencing reveals evolution of immune landscape during glioblastoma progression
Tufts University · Beth Israel Deaconess Medical Center · +3 more institutions
Abstract
Abstract Glioblastoma (GBM) is an incurable primary malignant brain cancer hallmarked with a substantial protumorigenic immune component. Knowledge of the GBM immune microenvironment during tumor evolution and standard of care treatments is limited. Using single-cell transcriptomics and flow cytometry, we unveiled large-scale comprehensive longitudinal changes in immune cell composition throughout tumor progression in an epidermal growth factor receptor-driven genetic mouse GBM model. We identified subsets of proinflammatory microglia in developing GBMs and anti-inflammatory macrophages and protumorigenic myeloid-derived suppressors cells in end-stage tumors, an evolution that parallels breakdown of the…
Citation impact
- FWCI
- 22.93
- Percentile
- 100%
- References
- 84
Authors
13- ATAlan T. YeoCorresponding
Tufts University, Beth Israel Deaconess Medical Center, Harvard University
- SRShruti Rawal
Beth Israel Deaconess Medical Center, Harvard University
- BDBethany Delcuze
Tufts University, Beth Israel Deaconess Medical Center, Harvard University
- ACAnthos Christofides
Beth Israel Deaconess Medical Center, Harvard University
- AMAgata M. P. Atayde
Beth Israel Deaconess Medical Center, Harvard University
Topics & keywords
- Immune system
- Biology
- Cancer research
- Microglia
- CD8
- Tumor microenvironment
- Temozolomide
- Tumor progression