Single-cell RNA-seq analysis reveals BHLHE40-driven pro-tumour neutrophils with hyperactivated glycolysis in pancreatic tumour microenvironment
Shanghai Jiao Tong University · Ruijin Hospital · +5 more institutions
Abstract
Objective Innate immunity plays important roles in pancreatic ductal adenocarcinoma (PDAC), as non-T-cell-enriched tumour. Neutrophils are major players in innate immune system. Here, we aimed to explore the heterogeneity and pro-tumour mechanisms of neutrophils in PDAC. Design We analysed single-cell transcriptomes of peripheral blood polymorphonuclear leucocytes (PMNs) and tumour-infiltrating immune cells from five patients with PDAC, and performed immunofluorescence/immunohistochemistry staining, multi-omics analysis and in vitro experiments to validate the discoveries of bioinformatics analysis. Results Exploration of the heterogeneity of tumour-associated neutrophils (TANs) revealed a terminally…
Citation impact
- FWCI
- 23.85
- Percentile
- 100%
- References
- 77
Authors
25- LWLiwen Wang
Shanghai Jiao Tong University, Ruijin Hospital, State Key Laboratory of Oncogene and Related Genes
- YLYihao Liu
Shanghai Jiao Tong University, Ruijin Hospital, State Key Laboratory of Oncogene and Related Genes
- YDYuting Dai
Shanghai Jiao Tong University, Ruijin Hospital, Shanghai Institute of Hematology
- XTXiaomei Tang
Shanghai Jiao Tong University, Ruijin Hospital, State Key Laboratory of Oncogene and Related Genes
- YTYin Tong
Shanghai Jiao Tong University, Ruijin Hospital, Shanghai Institute of Hematology
Topics & keywords
- Biology
- Transcriptome
- Innate immune system
- Glycolysis
- Tumor microenvironment
- Immune system
- Cancer research
- Population
- No poverty