METTL3 Inhibits Antitumor Immunity by Targeting m6A-BHLHE41-CXCL1/CXCR2 Axis to Promote Colorectal Cancer
Chinese University of Hong Kong · Chinese University of Hong Kong, Shenzhen · +3 more institutions
Abstract
Background & AimsN6-Methyladenosine (m6A) is the most prevalent RNA modification and recognized as an important epitranscriptomic mechanism in colorectal cancer (CRC). We aimed to exploit whether and how tumor-intrinsic m6A modification driven by methyltransferase like 3 (METTL3) can dictate the immune landscape of CRC.MethodsMettl3 knockout mice, CD34+ humanized mice, and different syngeneic mice models were used. Immune cell composition and cytokine level were analyzed by flow cytometry and Cytokine 23-Plex immunoassay, respectively. M6A sequencing and RNA sequencing were performed to identify downstream targets and pathways of METTL3. Human CRC specimens (n = 176) were used to evaluate correlation between…
Citation impact
- FWCI
- 22.18
- Percentile
- 100%
- References
- 34
Authors
17- HCHuarong Chen
Chinese University of Hong Kong, Chinese University of Hong Kong, Shenzhen
- YPYasi Pan
Chinese University of Hong Kong, Chinese University of Hong Kong, Shenzhen
- QZQiming Zhou
Chinese University of Hong Kong, Chinese University of Hong Kong, Shenzhen
- CLCong Liang
Xiamen University
- CWChi-Chun Wong
Chinese University of Hong Kong, Chinese University of Hong Kong, Shenzhen
Topics & keywords
- Colorectal cancer
- CXCL1
- Medicine
- Immunity
- Oncology
- Cancer
- Internal medicine
- Immunology