Spatially resolved multi-omics deciphers bidirectional tumor-host interdependence in glioblastoma

Glioblastomas are malignant tumors of the central nervous system hallmarked by subclonal diversity and dynamic adaptation amid developmental hierarchies. The source of dynamic reorganization within the spatial context of these tumors remains elusive. Here, we characterized glioblastomas by spatially resolved transcriptomics, metabolomics, and proteomics. By deciphering regionally shared transcriptional programs across patients, we infer that glioblastoma is organized by spatial segregation of lineage states and adapts to inflammatory and/or metabolic stimuli, reminiscent of the reactive transformation in mature astrocytes. Integration of metabolic imaging and imaging mass cytometry uncovered locoregional…

• DF
  Deutsche Forschungsgemeinschaft

  Award: SFB 824C04
• BF
  Bundesministerium für Bildung und Forschung

  Awards: 031L0260B, FMT 13GW0230A, 13GW0230A
• EK
  Else Kröner-Fresenius-Stiftung

  Award: 031L0260B
• FK
  Fördergemeinschaft Kinderkrebs-Zentrum Hamburg