Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery

Abstract Reactive oxygen species (ROS) play vital roles in intestinal inflammation. Therefore, eliminating ROS in the inflammatory site by antioxidant enzymes such as catalase and superoxide dismutase may effectively curb inflammatory bowel disease (IBD). Here, Escherichia coli Nissle 1917 (ECN), a kind of oral probiotic, was genetically engineered to overexpress catalase and superoxide dismutase (ECN-pE) for the treatment of intestinal inflammation. To improve the bioavailability of ECN-pE in the gastrointestinal tract, chitosan and sodium alginate, effective biofilms, were used to coat ECN-pE via a layer-by-layer electrostatic self-assembly strategy. In a mouse IBD model induced by different chemical drugs,…

• NN
  National Natural Science Foundation of China

  Awards: 21927803, 91959104, 51903182, 52032008
• MO
  Ministry of Education of the People's Republic of China

  Award: 2021YFF0701800
• CP
  China Postdoctoral Science Foundation
• GO
  Government of Jiangsu Province
• NS
  Natural Science Foundation of Jiangsu Province

  Award: BK20190826
• CI
  Collaborative Innovation Center of Suzhou Nano Science and Technology
• PS
  Postdoctoral Science Foundation of Jiangsu Province