Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics
South China Normal University · Hubei Provincial Center for Disease Control and Prevention
Abstract
CRISPR diagnostics based on nucleic acid amplification faces barriers to its commercial use, such as contamination risks and insufficient sensitivity. Here, we propose a robust solution involving optochemical control of CRISPR RNA (crRNA) activation in CRISPR detection. Based on this strategy, recombinase polymerase amplification (RPA) and CRISPR-Cas12a detection systems can be integrated into a completely closed test tube. crRNA can be designed to be temporarily inactivated so that RPA is not affected by Cas12a cleavage. After the RPA reaction is completed, the CRISPR-Cas12a detection system is activated under rapid light irradiation. This photocontrolled, fully closed CRISPR diagnostic system avoids…
Citation impact
- FWCI
- 18.28
- Percentile
- 100%
- References
- 73
Authors
6Topics & keywords
- Trans-activating crRNA
- CRISPR
- Recombinase Polymerase Amplification
- Nucleic acid
- Computational biology
- RNA
- Nucleic acid detection
- Chemistry
- Good health and well-being